Live 4D Imaging of the Embryonic Vertebrate Heart with Two-Photon Light Sheet Microscopy and Simultaneous Optical Phase Stamping
نویسندگان
چکیده
(CCFT-SXT). Correlation of these two three-dimensional datasets was performed using fiducial markers visible in both modalities. The Xi was identified in each cell by its strong enrichment in a fluorescently-labeled histone variant, macroH2A-EGFP. We present the overall topology, compaction profile, and volume occupied by the Xi in interphase nuclei. Physical contacts with nucleoli and the nuclear envelope are also described. Examination of eleven individual cells showed that there is considerable variation in the spatial arrangement and compaction profile adopted by the Xi.
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